Stat6 Antibody (AS6-45.13.9)recommended for detection of Stat6 of human origin by WB and IP

Stat6 Antibody (AS6-45.13.9): sc-53611

Stat6 Antibody (AS6-45.13.9) is rated 3.5 out of 5 by 2.
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Datasheets

    • Stat6 Antibody (AS6-45.13.9) is a Armenian hamster monoclonal IgG provided at 200 µg/ml
    • raised against Stat6 of human origin
    • recommended for detection of Stat6 of human origin by WB and IP; may cross-react with Stat1, Stat3, or Stat4
    • See Stat6 (D-1): sc-374021 for Stat6 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using Stat6 (AS6-45.13.9): sc-53611?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from A little messy and faint in K A little messy and faint in K-562, HeLa and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-01-08
Rated 4 out of 5 by from Good strong bands in human transfected lysates Good strong bands in human transfected lysates. -SCBT QC
Date published: 2014-03-05
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