SSEA-4 Antibody (MC813)recommended for detection of SSEA-4 of mouse, rat and human origin by IF and FCM

SSEA-4 Antibody (MC813): sc-59368

SSEA-4 Antibody (MC813) is rated 4.7 out of 5 by 3.
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Datasheets

    • SSEA-4 Antibody (MC813) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • raised against embryonal carcinoma cell line 2102Ep of human origin
    • recommended for detection of SSEA-4 of mouse, rat and human origin by IF and FCM; non cross-reactive with undifferentiated murine EC, ES and EG cells
    • available conjugated to phycoerythrin for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See SSEA-4 (813-70): sc-21704 for SSEA-4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can SSEA-4 (MC813): sc-59368 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SSEA-4 (MC813): sc-59368 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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