SSEA-4 Antibody (813-70)recommended for detection of SSEA-4 of mouse, rat and human origin by IF and FCM

SSEA-4 Antibody (813-70): sc-21704

SSEA-4 Antibody (813-70) is rated 4.9 out of 5 by 8.
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Datasheets

    • SSEA-4 Antibody (813-70) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • raised against human embryonal carcinoma cell line 2102Ep
    • recommended for detection of SSEA-4 of mouse, rat and human origin by IF and FCM; non cross-reactive with undifferentiated murine EC, ES and EG cells
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SSEA-4 (813-70): sc-21704.
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I would like to detect SSEA-4 by flow cytometer. Does this antibody (sc-21704 PE) contain conjugated dye which would emit the step of incubation with secondary antibody? Which isotype control should I use for this antibody (sc-21704 PE)?

Asked by: arup
Thank you for your question. Yes, we offer this antibody directly conjugated to phycoerythrin (sc-21704 PE), which would allow for direct fluorescent detection without the use of a secondary antibody. SSEA-4 Antibody (813-70) is a mouse monoclonal IgG3. If you are using the PE conjugated primary antibody, the recommended isotype control is normal mouse IgG3-PE: sc-2869. A link is provided below as a reference. https://www.scbt.com/scbt/product/normal-mouse-igg3-pe
Answered by: Technical Support
Date published: 2017-10-25

Which negative control should be used when using the FITC conjugated SSEA-4 (813-70): sc-21704 in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using isotype specific normal mouse IgG3 conjugated to FITC, sc-2858
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from SSEA4 We used it for immunofluorescence in human ESC and iPSC. It worked ok!
Date published: 2018-11-23
Rated 5 out of 5 by from Result I use it for IF.My experiment material is human induced pluripotent stem cells,antibody dilution ratio is 1:50,but my experiment failed.Thank you for your provide samples, I'll try to find the reason and do it again.
Date published: 2017-09-06
Rated 4 out of 5 by from Great product Both fcm and ihc results were very strong; pleased
Date published: 2017-01-02
Rated 5 out of 5 by from Used for Flow Cytometry with human ESCs Used for Flow Cytometry with human ESCs. -SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by from Published IF data using hESC lines HUES Published IF data using hESC lines HUES-5 (H5) & WA09(H9). -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from ICC: human skin biopsies ICC: human skin biopsies. -SCBT Publication Review
Date published: 2015-04-21
Rated 5 out of 5 by from Worked well for immunohistochemical analysis Worked well for immunohistochemical analysis of SSEA-4 in human embryonic stem cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Produced excellent indirect FCM data for Produced excellent indirect FCM data for live NTERA-2 cl.D1 cells stained with SSEA-4 . -SCBT QC
Date published: 2014-07-12
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