SSEA-1 Antibody (480)recommended for detection of SSEA-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

SSEA-1 Antibody (480): sc-21702

SSEA-1 Antibody (480) is rated 5.0 out of 5 by 11.
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Datasheets

    • SSEA-1 Antibody (480) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against X-irradiated F9 teratocarcinoma stem cells
    • recommended for detection of SSEA-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • available conjugated to either PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SSEA-1 (480): sc-21702.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What exactly does it mean that this antibody is "monoclonal mouse IgM κ (kappa light chain)" antibody? Is there no heavy chain present in this antibody molecule? Can standard anti-mouse IgM secondary antibodies recognize this IgM κ antibody?

Asked by: TSaric
Thank you for your question. SSEA-1 (480): sc-21702 is a complete monomeric IgM antibody with heavy and light chains. It can be detected either by regular anti-mouse IgM antibody or by using one of our recommended κ light chain-specific secondary binding proteins, such as m-IgGκ BP-HRP sc-516102, or one of our CruzFluor conjugated κ binding proteins. You can find all available options by following this link: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What secondary antibody may I use for WB? The antibody sc-21702 is a IgM Light chain.

Asked by: pablo
Thank you for your question. We would suggest using our mouse IgG kappa binding protein secondary reagent (sc-516102) for an IgM antibody. If you would like to try a sample of the reagent, please contact your local distributor. You can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-06-20

What secondary reagent should I use if I want to perform IF studies in mice tissue samples?

Asked by: Trav11
In this case, we recommend using one of our directly conjugated mouse monoclonal primary antibodies. Currently, SSEA-1 Antibody (480): sc-21702 is available directly conjugated to HRP (sc-21702 HRP), phycoerythrin (sc-21702 PE), fluorescein (sc-21702 FITC), Alexa Fluor® 488 (sc-21702 AF488), Alexa Fluor® 647 (sc-21702 AF647), PerCP (sc-21702 PerCP) or Alexa Fluor® 405 (sc-21702 AF405). If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 5 out of 5 by from Parfait ! Antibody tested on paraffin section (mouse embryonic gonad 12.5 days post coitum). This antibody perfectly detects germ cells. The membrane signal is perfect and there is no background noise. Conditions:   - Demasking with Pt Link from Dako (pH6 or pH9)   - Dilution 1/500 and 1/200 works well   - Secondary antibody used: Alexa anti-mouse 647
Date published: 2018-06-15
Rated 5 out of 5 by from Beautiful staining Wonderful staining seen in IHC and IF with both methanol fixation and FFPE sections.
Date published: 2017-01-12
Rated 5 out of 5 by from Worked well with mIPS cells by IF To detect the expression of SSEA-1 in miPS cells by IF, I bought this antibody. The cells were incubated with sc-21702 at a 1/500 dilution overnight at +4°C, and found this antibody worked well.
Date published: 2016-12-06
Rated 5 out of 5 by from 抗体效价很高,使用效果很好,使用干细胞染色分群很开 几个月前购买的该抗体,作为流式细胞分选和分析用。使用该抗体进行多能干细胞染色效果很好用,分群比较开。
Date published: 2016-11-17
Rated 5 out of 5 by from It's woeked well in IF It's woeked well in IF
Date published: 2015-11-17
Rated 5 out of 5 by from Used for studying adult stem cell/pluripotency Used for studying adult stem cell/pluripotency
Date published: 2015-05-28
Rated 5 out of 5 by from Worked for western blotting in MEF cells Worked for western blotting in MEF cells. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by from Published immunofluorescence data using murine Published immunofluorescence data using murine ES: embryonic stem cells. -SCBT Publication Review
Date published: 2015-04-05
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