Anti-Squalene synthetase Antibody (A-7) recommended for detection of Squalene synthetase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Squalene synthetase Antibody (A-7): sc-271602

Anti-Squalene synthetase Antibody (A-7) is rated 4.5 out of 5 by 4.
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Datasheets

    • Anti-Squalene synthetase Antibody (A-7) is a mouse monoclonal IgG1 κ Squalene synthetase antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Squalene synthetase of human origin
    • Anti-Squalene synthetase Antibody (A-7) is recommended for detection of Squalene synthetase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Squalene synthetase Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Squalene synthetase (A-7): sc-271602.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Squalene synthetase Antibody (A-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Squalene synthetase Antibody (A-7) (see ordering information below).
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想问一下这个分子量是多少啊

Asked by: huling
Thank you for your question. The expected molecular weight for Squalene synthetase is 52 kDa. 感谢您的问题。Squalene synthetase的预期分子量是52 kDa。
Answered by: Technical Support 9
Date published: 2019-08-20

Can Squalene synthetase (A-7): sc-271602 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Squalene synthetase (A-7): sc-271602 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Clear band in WB Clear band in WB of KM12 and Hela cells (50 ug):1:400 in 5% Milk/TBST.
Date published: 2018-11-02
Rated 4 out of 5 by from Good Poor signals, used 1:300 dilution in western blot !!
Date published: 2018-02-07
Rated 5 out of 5 by from Very good Antibody for WB 1:400 dilution can detect non-transfected FDFT1(Squalene synthetase) in Hela, SHEP1, 293FT.
Date published: 2016-11-14
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