SQSTM1 Antibody (A-6)recommended for detection of SQSTM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SQSTM1 Antibody (A-6): sc-48402

SQSTM1 Antibody (A-6) is rated 4.5 out of 5 by 17.
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Datasheets

    • SQSTM1 Antibody (A-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 151-440 of SQSTM1 of human origin
    • SQSTM1 Antibody (A-6) is recommended for detection of SQSTM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • SQSTM1 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SQSTM1 (A-6): sc-48402.
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I am using SQSTM1 (A-6): sc-48402 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from cannot detect the band I used this antibody to detect protein but I cannot see 62kDa band. It was so strange even i changed to other cell lines. there are a numerous non- specific band and I got a very strong signal at appox. 99kDa position. I really cannot understand this situation.
Date published: 2018-07-20
Rated 3 out of 5 by from non specific bands in WB I used this antibody in WB experiments in human fibroblasts. I got a lot of non specific bands in other places.
Date published: 2018-06-06
Rated 5 out of 5 by from Works well I recently received this antibody in the Hinck Lab, and I am using it for WB. The antibody shows great detection in WB analyses, and no background.
Date published: 2018-05-09
Rated 5 out of 5 by from Nice band We checked the antibody in MDCK cells (canine). The conditions were: - 50 u g protein per lane - 1:1,000 dilution in BSA (1% in TBST) - incubation overnight at 4ºC with agitation - secondary antibody: 1:10,000 dilution in BSA (1% TBST)
Date published: 2018-04-09
Rated 3 out of 5 by from Ok signal in mousce tissue OK signal in mouse muscle tissue. No signal in mouse muscle cells.
Date published: 2018-02-22
Rated 5 out of 5 by from High specificity with dilutions around 1:200 This antibody works well in Western blot (1:200) for mouse brain in alcoholism model
Date published: 2017-12-25
Rated 5 out of 5 by from WB结果很好,价格也很实在 用了其它几个知名品牌的产品,WB做不出来,用Santa 的效果很好,得到了满意的结果,抗体4度保存,取用方便。
Date published: 2017-12-04
Rated 4 out of 5 by from 条带清楚 条带清楚, 但是有杂带 感觉特异性上还差一点 不过可以用啦。希望以后能够多出一点其他种类的抗体,可以用。
Date published: 2017-09-22
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