SPRED1 Antibody (462CT2.5.1): sc-517362
- SPRED1 Antibody (462CT2.5.1) is a mouse monoclonal IgG1 provided at 100 µg/ml
- raised against purified His-tagged SPRED1 protein fragment of human origin
- recommended for detection of SPRED1 of mouse, rat and human origin by WB
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SPRED1 Antibody (462CT2.5.1). This work is in progress.
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SPRED1 Antibody (462CT2.5.1) is a mouse monoclonal IgG1 antibody that detects SPRED1 in mouse, rat, and human samples by western blotting (WB). SPRED1, also known as NFLS, is a 444 amino acid protein characterized by one KBD domain, one sprouty domain, and one WH1 domain, which are crucial for regulatory functions. SPRED1 is primarily localized to the cell membrane and cholesterol-rich membrane raft fractions, where SPRED1 plays a vital role as a tyrosine kinase that modulates the activity of the ERK (extracellular signal-regulated kinase) cascade. By inhibiting growth-factor-mediated activation of ERK, SPRED1 is essential for controlling cellular responses to growth signals, thereby influencing processes such as cell proliferation and differentiation. SPRED1 can function independently as a homodimer or in conjunction with SPRED2, further highlighting SPRED1′s importance in cellular signaling pathways. Mutations in the SPRED1 gene are linked to neurofibromatosis type 1-like syndrome (NFLS), an autosomal dominant condition marked by multiple café-au-lait spots, axillary freckling, and macrocephaly, underscoring SPRED1′s clinical significance in human health.
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SPRED1 Antibody (462CT2.5.1) References:
- Spred1 is required for synaptic plasticity and hippocampus-dependent learning. | Denayer, E., et al. 2008. J Neurosci. 28: 14443-9. PMID: 19118178
- SPRED1 germline mutations caused a neurofibromatosis type 1 overlapping phenotype. | Pasmant, E., et al. 2009. J Med Genet. 46: 425-30. PMID: 19366998
- miR126 positively regulates mast cell proliferation and cytokine production through suppressing Spred1. | Ishizaki, T., et al. 2011. Genes Cells. 16: 803-14. PMID: 21668589
- NMR resonance assignments of the EVH1 domain of neurofibromin's recruitment factor Spred1. | Führer, S., et al. 2017. Biomol NMR Assign. 11: 305-308. PMID: 28831766
- Structural Insights into the SPRED1-Neurofibromin-KRAS Complex and Disruption of SPRED1-Neurofibromin Interaction by Oncogenic EGFR. | Yan, W., et al. 2020. Cell Rep. 32: 107909. PMID: 32697994
- A Study on the Expression of SPRED1 and PBRM1 (Baf180) and their Clinical Significances in Patients with Gastric Cancer. | Liu, W., et al. 2020. Clin Lab. 66: PMID: 33073945
- miR-126-3p contributes to sorafenib resistance in hepatocellular carcinoma via downregulating SPRED1. | Tan, W., et al. 2021. Ann Transl Med. 9: 38. PMID: 33553331
- MEK inhibition ameliorates social behavior phenotypes in a Spred1 knockout mouse model for RASopathy disorders. | Borrie, SC., et al. 2021. Mol Autism. 12: 53. PMID: 34311771
- Hypoxic Bone Marrow Stromal Cells Secrete miR-140-5p and miR-28-3p That Target SPRED1 to Confer Drug Resistance in Multiple Myeloma. | Zhang, H., et al. 2024. Cancer Res. 84: 39-55. PMID: 37756570
- Legius syndrome mutations in the Ras-regulator SPRED1 abolish its membrane localization and potentially cause neurodegeneration. | Hirata, Y., et al. 2024. J Biol Chem. 300: 107969. PMID: 39510187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPRED1 Antibody (462CT2.5.1) | sc-517362 | 100 µg/ml | $322.00 |