Spi-B Antibody (4G5B3): sc-517204

Spi-B Antibody (4G5B3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Spi-B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-Spi-B antibody (4G5B3) is available as the non-conjugated format. Spi-B, a member of the Ets transcription factor family, plays a crucial role in regulating gene expression during lymphoid development, particularly in B cells and immature T cells. A winged helix-turn-helix motif known as the Ets domain allows DNA sequence binding, influencing transcription of genes essential for immune function. Spi-B enhances expression of several B cell genes, including those encoding CD72, CD20, and Btk, which are vital for B cell activation and differentiation. While PU.1, another Ets family member, expresses across various hematopoietic cells, Spi-B expression remains more restricted, accumulating during T-lineage commitment and diminishing significantly after beta-selection checkpoint. This specificity underscores Spi-B′s importance in immune system development and function, making Spi-B (4G5B3) monoclonal antibody an invaluable tool for researchers studying lymphocyte biology and related disorders.

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Spi-B Antibody (4G5B3) References:

1. Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development.  |  Bemark, M., et al. 1999. J Biol Chem. 274: 10259-67. PMID: 10187812
2. PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction.  |  Garrett-Sinha, LA., et al. 1999. Immunity. 10: 399-408. PMID: 10229183
3. Precise developmental regulation of Ets family transcription factors during specification and commitment to the T cell lineage.  |  Anderson, MK., et al. 1999. Development. 126: 3131-48. PMID: 10375504
4. The Ets factors PU.1 and Spi-B regulate the transcription in vivo of P2Y10, a lymphoid restricted heptahelical receptor.  |  Rao, S., et al. 1999. J Biol Chem. 274: 34245-52. PMID: 10567398
5. Delta-like1-induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 and Spi-B.  |  Dontje, W., et al. 2006. Blood. 107: 2446-52. PMID: 16317090
6. The mechanism of action of Spi-B in the transcriptional activation of the interferon-α4 gene.  |  Miyazaki, R., et al. 2020. Biochem Biophys Res Commun. 525: 477-482. PMID: 32111355
7. Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia.  |  Ariga, Y., et al. 2022. J Histochem Cytochem. 70: 683-694. PMID: 36169277
8. TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation.  |  Zhao, J., et al. 2023. J Autoimmun. 139: 103087. PMID: 37481835
9. PU.1 (Spi-1) autoregulates its expression in myeloid cells.  |  Chen, H., et al. 1995. Oncogene. 11: 1549-60. PMID: 7478579
10. The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation.  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
11. Neutrophils and monocytes express high levels of PU.1 (Spi-1) but not Spi-B.  |  Chen, HM., et al. 1995. Blood. 85: 2918-28. PMID: 7742552
12. The Ets protein Spi-B is expressed exclusively in B cells and T cells during development.  |  Su, GH., et al. 1996. J Exp Med. 184: 203-14. PMID: 8691135

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Spi-B Antibody (4G5B3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Spi-B Antibody (4G5B3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Spi-B Antibody (4G5B3) Specifications

Alternate Product Name: Spi-B

Product Clone Name: 4G5B3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 200-252

Epitope Target: Spi-B

Epitope Species: human

Product Reactivity/Detection: Spi-B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Spi-B Antibody (4G5B3): N/A