Anti-SphK2 Antibody (9C5E1) recommended for detection of SphK2 of human origin by IF, IHC(P), FCM and ELISA

Anti-SphK2 Antibody (9C5E1): sc-517192

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Datasheets

    • Anti-SphK2 Antibody (9C5E1) is a mouse monoclonal IgG1 κ SphK2 antibody, cited in 1 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to amino acids 36-52 of SphK2 of human origin
    • recommended for detection of SphK2 of human origin by IF, IHC(P), FCM and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SphK2 Antibody (9C5E1). This work is in progress.
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Can sc-517192: SphK2 (9C5E1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-517192: SphK2 (9C5E1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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