SOD-2 Antibody (24)recommended for detection of SOD-2 of human origin by WB, IP and ELISA

SOD-2 Antibody (24): sc-130346

SOD-2 Antibody (24) is rated 4.0 out of 5 by 1.
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Datasheets

    • SOD-2 Antibody (24) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant SOD-2 of human origin
    • recommended for detection of SOD-2 of human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See SOD-2 (E-10): sc-137254 for SOD-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using SOD-2 (24): sc-130346?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Strong Strong, clear band in WB in DU 145 whole cell lysate. -SCBT QC
Date published: 2013-08-23
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