SOD-1 Antibody (24)recommended for detection of SOD-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SOD-1 Antibody (24): sc-101523

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Datasheets

    • SOD-1 Antibody (24) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full-length recombinant SOD-1 of human origin
    • SOD-1 Antibody (24) is recommended for detection of SOD-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • SOD-1 Antibody (24) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SOD-1 (24): sc-101523.
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I am using SOD-1 (24): sc-101523 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-11-15
  • y_2019, m_12, d_4, h_22CST
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Rated 5 out of 5 by from super strong Antibody detects single extremely strong band at around 17kDa in U2OS lysates in WB. Dilution 1:2000. I would recommend higher dilution, like 1:3000-4000
Date published: 2019-10-22
Rated 1 out of 5 by from No nos ha funcionado. Probado en lisados de celulas AR42J, no hemosd etectado ninguna banda.
Date published: 2019-05-09
Rated 5 out of 5 by from Good results Used in PMS from primary neuronal culture lysate, reallly good and reproducible results.
Date published: 2019-03-12
Rated 5 out of 5 by from Very good in WB Application: Western blotting Dilution 1:1000 Cell lysate of C2C12 Very strong and specific band
Date published: 2018-10-05
Rated 4 out of 5 by from good results in WB Good results in Western Blot, we used it 1:500 in milk
Date published: 2018-05-16
Rated 5 out of 5 by from 效果不错 一个月前买的这个产品,效果很好,下次继续买这家的。我还会推荐别人买,好东西大家都支持,圣克鲁斯加油!
Date published: 2018-04-04
Rated 4 out of 5 by from the only SOD1 that worked tested SOD1 monoclonal ab (sc-101523 (1:1000), sc-515404 (1:100) and sc-271014 (1:100)) in western blots with 100 ug protein lysates from rat heart or rat artery transferred onto nitrocellulose membranes probed with LiCor secondary abs. only SOD1: sc-101523 produced a distinct band at expected kD.
Date published: 2017-11-17
Rated 5 out of 5 by from Nice SOD1 detection in Jurkat cells We used this antibody for expression detection of SOD1 by western blot. We got a nice band around the correct molecular weight in Jurkat cells.
Date published: 2017-02-07
  • y_2019, m_12, d_3, h_22
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