SNAP47 Antibody (D-11)recommended for detection of SNAP47 of human origin by WB, IP, IF and ELISA

SNAP47 Antibody (D-11): sc-514428

SNAP47 Antibody (D-11) is rated 4.5 out of 5 by 4.
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Datasheets

    • SNAP47 Antibody (D-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 290-442 mapping near the C-terminus of SNAP47 of human origin
    • SNAP47 Antibody (D-11) is recommended for detection of SNAP47 of human origin by WB, IP, IF and ELISA
    • SNAP47 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNAP47 (D-11): sc-514428.
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What is the recommended fixation method for immunofluorescence staining with SNAP47 (D-11): sc-514428 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from well done it works well in sk-hep-1 cell by 1:1000 in WB,thanks!
Date published: 2017-11-04
Rated 5 out of 5 by from Antibody works! Antibody detected a 47 kDa protein after immunoprobing overnight (1:1000 dilution in 2.5% TBST). Signal seems to be sensitive to my experimental treatments as well. Hoping to further confirm with siSNAP47 experiments.
Date published: 2017-08-25
Rated 4 out of 5 by from not for hydra Tested in IHC in hydra vulgaris and did not react, as expected. Did not try in any other species
Date published: 2017-08-01
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