Anti-SMN Antibody (MANSMA1) recommended for detection of SMN of mouse, rat and human origin by WB, IP and IF

Anti-SMN Antibody (MANSMA1): sc-81555

Anti-SMN Antibody (MANSMA1) is rated 4.0 out of 5 by 2.
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Datasheets

    • SMN Antibody (MANSMA1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length recombinant SMN of human origin, with epitope mapping to amino acids 28-91
    • recommended for detection of SMN of mouse, rat and human origin by WB, IP and IF
    • See SMN (2B1): sc-32313 for SMN antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SMN Antibody (MANSMA1) for WB applications. These reagents are now offered in bundles with SMN Antibody (MANSMA1) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-81555: SMN (MANSMA1) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC
Date published: 2015-04-22
Rated 4 out of 5 by from Strong band in WB in K Strong band in WB in K-562 and HepG2 whole cell lysates. -SCBT QC
Date published: 2014-04-21
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