Anti-SMN Antibody (H-7) recommended for detection of SMN of human origin by WB, IP, IF and ELISA

Anti-SMN Antibody (H-7): sc-137200

Anti-SMN Antibody (H-7) is rated 5.0 out of 5 by 4.
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Datasheets

    • SMN Antibody (H-7) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-195 mapping near the N-terminus of SMN of human origin
    • recommended for detection of SMN of human origin by WB, IP, IF and ELISA
    • See SMN (2B1): sc-32313 for SMN antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with SMN (H-7): sc-137200 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell lysate Good specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-07-13
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Strong band in WB in 293T whole cell lysate and K Strong band in WB in 293T whole cell lysate and K-562 nuclear extract. -SCBT QC
Date published: 2015-02-17
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-16
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