Anti-SMN Antibody (11F3) recommended for detection of SMN of mouse, rat and human origin by WB, IP and IF

Anti-SMN Antibody (11F3): sc-81554

Anti-SMN Antibody (11F3) is rated 4.0 out of 5 by 2.
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Datasheets

    • SMN Antibody (11F3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length recombinant SMN of human origin, with epitope mapping to amino acids 28-91
    • recommended for detection of SMN of mouse, rat and human origin by WB, IP and IF
    • See SMN (2B1): sc-32313 for SMN antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SMN Antibody (11F3) for WB applications. These reagents are now offered in bundles with SMN Antibody (11F3) (see ordering information below).
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For Western Blot, is it recommended to use denatured or non-denatured conditions with SMN (11F3): sc-81554 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-06-21
Rated 4 out of 5 by from Strong band in WB in HeLa and SK Strong band in WB in HeLa and SK-BR-3 whole cell lysates. -SCBT QC
Date published: 2014-11-11
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