Smad SBE Gel Shift Oligonucleotides
  • SBE binding site for Smad transcription factors; supplied as 500 ng double-stranded DNA; sc-2603
  • also available as mutant oligonucleotide with "GTCTAGAC"->"CATAGCGT" substitution in Smad SBE binding motif; sc-2604
  • 5'-G TCT AGA C CA-3'

Smad SBE Gel Shift Oligonucleotides: sc-2603

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Datasheets

    • SBE binding site for Smad transcription factors; supplied as 500 ng double-stranded DNA; sc-2603
    • also available as mutant oligonucleotide with "GTCTAGAC"->"CATAGCGT" substitution in Smad SBE binding motif; sc-2604
    • 5'-G TCT AGA C CA-3'
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I want to know this oligonucleotides is for all homologous Smad (such as smad1,smad2,smad3......). I need to detect the binding activity of Smad2/3,could I use this product? If no, what else product for Smad2/3 gel shift oligonucleotides product I can buy

Asked by: Hailen
Thank you for your question. This product, Smad SBE Gel Shift Oligonucleotides: sc-2603, is derived from the Smad Binding Element (SBE) as described in the literature (PMID: 16183640). Based on more recent literature we found, the Smad SBE is suitable for binding activity of Smad 3 and Smad 4, "an 8-bp palindromic DNA sequence, GTCTAGAC, was identified as a Smad3 and Smad4 binding motif. In contrast to Smad3 and Smad4, Smad2 does not directly bind to DNA due to steric hindrance by an inserted sequence in the DNA-binding region." See the reference below for more information. Morikawa M, Koinuma D, Miyazono K, Heldin C-H. Genome-wide mechanisms of Smad binding. Oncogene. 2013;32(13):1609-1615. doi:10.1038/onc.2012.191.
Answered by: Technical Support
Date published: 2017-02-03
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