Skp2 p45 Antibody (A-2)recommended for detection of Skp2 p45 of mouse, rat and human origin by WB, IP, IF and ELISA

Skp2 p45 Antibody (A-2): sc-74477

Skp2 p45 Antibody (A-2) is rated 4.2 out of 5 by 20.
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Datasheets

    • Skp2 p45 Antibody (A-2) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-435 representing full length Skp2 p45 of human origin
    • Skp2 p45 Antibody (A-2) is recommended for detection of Skp2 p45 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Skp2 p45 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-74477 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Skp2 p45 (A-2): sc-74477.
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When should the TransCruz reagent sc-74477 X be used instead of the standard form sc-74477?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from sc-74477 worked okay for murine dendritic cells. Signals not bright enough
Date published: 2019-11-18
Rated 5 out of 5 by from SKP2 Aplin Lab used SKP2 antibody for western blotting.
Date published: 2019-02-06
Rated 5 out of 5 by from 抗体特异性好,效价高,很不错 skp2鼠单克隆抗体很好用,很少出现非特异性的杂带,效价比高,可用作WB,CO-IP,但用作免疫荧光效果欠佳
Date published: 2018-01-10
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were from human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from work well in WB ork well in WB at 1:100, skeletal muscle and C2C12 cells, mouse
Date published: 2017-11-03
Rated 4 out of 5 by from WB OF MY WORK We used this antibody for a western blot and got a nice specific band at the correct molecular weight
Date published: 2017-08-30
Rated 3 out of 5 by from Maybe not suitable for rat protein 1:100 diluted for wb of rat cardiac protein,the band is quite weak.
Date published: 2017-08-23
Rated 5 out of 5 by from nice Antibody titer is very low, miscellaneous with too much, you can consider and then improve。
Date published: 2017-07-05
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