Skp1 p19 Antibody (H-6)recommended for detection of Skp1 p19 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

Skp1 p19 Antibody (H-6): sc-5281

Skp1 p19 Antibody (H-6) is rated 4.4 out of 5 by 8.
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Datasheets

    • Skp1 p19 Antibody (H-6) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-163 of Skp1 p19 of human origin
    • Skp1 p19 Antibody (H-6) is recommended for detection of Skp1 p19 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and avian
    • Skp1 p19 Antibody (H-6) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Skp1 p19 (H-6): sc-5281.
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Can Skp1 p19 (H-6): sc-5281 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Skp1 p19 (H-6): sc-5281 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Did't work for endogenous protein in 293T cells The antibody did not work for detection of endogenous Skp1 in HEK 293T cells by western blot (dilution 1:1000).
Date published: 2018-10-25
Rated 3 out of 5 by from A good antibody We got this sample antibody to test detection of SKP1 in immunoblotting. A weak band was visible. It appears the specific band we expected.
Date published: 2017-04-20
Rated 5 out of 5 by from Excellent The antibody is clean and specific in Western blot
Date published: 2017-03-14
Rated 5 out of 5 by from High quality! The antibody works well, the samples are cell lysates, high sensitive.
Date published: 2016-10-27
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human breast tumor. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Fantastic WB data in A Fantastic WB data in A-431 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from IF publication with mouse SN4741 samples IF publication with mouse SN4741 samples. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-01-14
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