SIRP- alpha / beta Antibody (A-1)recommended for detection of SIRP-α and SIRP-β of human origin by WB, IP, IF, IHC(P) and ELISA

SIRP-α/β Antibody (A-1): sc-17803

SIRP-α/β Antibody (A-1) is rated 3.8 out of 5 by 5.
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Datasheets

    • SIRP-α/β Antibody (A-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of SIRP-α/β of human origin
    • SIRP- alpha / beta Antibody (A-1) is recommended for detection of SIRP-α and SIRP-β of human origin by WB, IP, IF, IHC(P) and ELISA
    • SIRP- alpha / beta Antibody (A-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRP-α/β (A-1): sc-17803.
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Can SIRP-α/β (A-1): sc-17803 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, SIRP-α/β (A-1): sc-17803 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-06-15
Rated 4 out of 5 by from Publishable data in IP using human smooth Publishable data in IP using human smooth muscle cells -SCBT Publication Review
Date published: 2015-05-12
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membrane Produced beautiful immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-09
Rated 3 out of 5 by from Robust bands seen in WB using THP Robust bands seen in WB using THP-, AML-193 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-02-16
Rated 4 out of 5 by from Publishable data in WB using human HEK293 cells Publishable data in WB using human HEK293 cells. -SCBT Publication Review
Date published: 2015-01-16
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