SIRP-alpha Antibody (SE7C2)recommended for detection of SIRP-α of human origin by WB, IP and FCM

SIRP-α Antibody (SE7C2): sc-23863

SIRP-α Antibody (SE7C2) is rated 4.6 out of 5 by 7.
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Datasheets

    • SIRP-α Antibody (SE7C2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant extracellular domain of human SIRP-α
    • SIRP-alpha Antibody (SE7C2) is recommended for detection of SIRP-α of human origin by WB, IP and FCM
    • SIRP-alpha Antibody (SE7C2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRP-α (SE7C2): sc-23863.
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Bonjour, Pourriez-vous me dire si cet anticorps est capable de lier les deux isoformes de SIRPa (SIRPa1 et SIRPa2) ou s'il est spécifique de l'un ou de l'autre? Merci.

Asked by: Magali
Merci pour votre requête. Cet anticorps ne réagit pas en Western Blot avec l'isoforme SIRPa2 mais est spécifique de l'isoforme SIRPa1. Thank you for your inquiry. This antibody does not react in Western Blot with isoform SIRPa2 but is specific for isoform SIRPa1.
Answered by: Technical Support Europe
Date published: 2017-01-27

Which negative control should be used when using the FITC conjugated SIRP-å (SE7C2): sc-23863 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using normal mouse IgG conjugated to FITC, sc-2339 as a negative control
Answered by: Technical Support 15
Date published: 2017-01-10

Bonjour, J'ai besoin de connaître la séquence du domaine/peptide contre lequel a été générée l'anticorps anti-SIRPa SE7C2. Pourriez-vous m'envoyer cette séquence s'il vous plaît ?

Asked by: Vanessa
Merci pour votre question. L'anticorps a été généré contre une protéine recombinante contenant le domaine extracellulaire de SIRPα1 d'origine humaine. http://www.uniprot.org/uniprot/P78324 Thank you for your question. The antibody was generated against a recombinant protein containing the entire extracellular domain of human SIRPα1. http://www.uniprot.org/uniprot/P78324
Answered by: Technical Support Europe
Date published: 2016-12-16
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Rated 5 out of 5 by from good quality I bought this antibody a month ago and tested in human macrophages derived from monocytes. It stained the cells as described.
Date published: 2017-03-27
Rated 5 out of 5 by from Strong FCM and western !! Pleased with both FCM and western blot results !!!
Date published: 2017-01-14
Rated 5 out of 5 by from Fantastic for FCM! The SIRP-alpha Antibody (SE7C2) works very well for flow cytometry studies with publication citation for verfication.
Date published: 2016-12-28
Rated 3 out of 5 by from Strong bands seen in WB using HL Strong bands seen in WB using HL-60, THP-1 and human peripheral blood whole cell lysates. -SCBT QC
Date published: 2015-07-09
Rated 5 out of 5 by from WB publishable data with human CD3+ T cells WB publishable data with human CD3+ T cells - SCBT Publication Review
Date published: 2015-06-09
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Exceptional FCM data of human peripheral Exceptional FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2013-03-04
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