SIRP-α Antibody (SE7C2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against recombinant extracellular domain of human SIRP-α
SIRP-alpha Antibody (SE7C2) is recommended for detection of SIRP-α of human origin by WB, IP and FCM
SIRP-alpha Antibody (SE7C2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SIRP-α (SE7C2): sc-23863.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
SIRPs (signal-regulatory proteins) are a family of transmembrane glycoproteins that were identified by their association with the Src homology 2 domain-containing protein-tyrosine phosphatase SHP-2 in response to insulin. The SIRP family negatively regulates the PI 3-kinase pathway, which may diminish EGFR-mediated motility and survival phenotypes that contribute to transformation of certain cell types. SIRP-α is a transmembrane protein which contains an extracellular portion with three immunoglobulin-like structures and a cytoplasmic region with four potential tyrosine phosphorylation sites. SIRP-α (also known as SIRP-α1, SIRP-α2 or SIRP-α3) is a substrate for activated receptor tyrosine kinases. In its tyrosine phosphorylated form, SIRP-α binds to SH-PTP2 through SH2 interactions and acts as an SH-PTP2 substrate. SIRP-α has been shown to have negative regulatory effects on cellular responses induced by growth factors, oncogenes and insulin. SIRP-β1 shares extensive sequence homology with SIRP-α in its extracellular portion but lacks the cytoplasmic portion. SIRP-γ, originally designated SIRP-β2 (SIRP-B2, CD172g) has unique characteristics from both the α and β versions. SIRP-γ is expressed on the majority of T cells and a proportion of B cells. CD47 associates with SIRP-γ, and this interaction signals unidirectionally only.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pourriez-vous me dire si cet anticorps est capable de lier les deux isoformes de SIRPa (SIRPa1 et SIRPa2) ou s'il est spécifique de l'un ou de l'autre?
Asked by: Magali
Merci pour votre requête. Cet anticorps ne réagit pas en Western Blot avec l'isoforme SIRPa2 mais est spécifique de l'isoforme SIRPa1.
Thank you for your inquiry. This antibody does not react in Western Blot with isoform SIRPa2 but is specific for isoform SIRPa1.
Answered by: Technical Support Europe
Date published: 2017-01-27
Which negative control should be used when using the FITC conjugated SIRP-å (SE7C2): sc-23863 monoclonal antibody in flow cytometry?
Asked by: jenni
Thank you for your inquiry. We recommend using normal mouse IgG conjugated to FITC, sc-2339 as a negative control
Answered by: Technical Support 15
Date published: 2017-01-10
J'ai besoin de connaître la séquence du domaine/peptide contre lequel a été générée l'anticorps anti-SIRPa SE7C2.
Pourriez-vous m'envoyer cette séquence s'il vous plaît ?
Asked by: Vanessa
Merci pour votre question. L'anticorps a été généré contre une protéine recombinante contenant le domaine extracellulaire de SIRPα1 d'origine humaine.
Thank you for your question. The antibody was generated against a recombinant protein containing the entire extracellular domain of human SIRPα1.
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