Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sigma Receptor Double Nickase Plasmid (m): sc-422068-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sigma Receptor Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sigma Receptor Double Nickase Plasmid (m) and Sigma Receptor Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sigmar1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sigma Receptor Antibody (B-5): sc-137075
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sigma Receptor Double Nickase Plasmid (m) | sc-422068-NIC | 20 µg | $410.00 |
Sigmar1 encodes the sigma receptor 1 (SIGMAR1), an endoplasmic reticulum membrane chaperone enriched at mitochondria-associated ER membranes that modulates Ca2+ exchange, ER stress signaling, and proteostasis. In mouse cells, SIGMAR1 influences mitochondrial bioenergetics and oxidative stress responses through its coupling to IP3R-dependent calcium flux and adaptive unfolded protein response pathways. The protein also interfaces with lipid metabolism and trafficking, shaping membrane microdomain organization and receptor signaling. Dysregulated SIGMAR1 activity has been linked in the literature to neurodegeneration, neuropathic pain, and metabolic stress phenotypes, making it a useful node for mechanistic studies of cellular homeostasis.
Sigma Receptor Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sigmar1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sigmar1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sigmar1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sigmar1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.