SGLT-2 Antibody (D-6)recommended for detection of SGLT-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SGLT-2 Antibody (D-6): sc-393350

SGLT-2 Antibody (D-6) is rated 4.6 out of 5 by 5.
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Datasheets

    • SGLT-2 Antibody (D-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 226-268 mapping within an internal region of SGLT-2 of human origin
    • SGLT-2 Antibody (D-6) is recommended for detection of SGLT-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • SGLT-2 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SGLT-2 (D-6): sc-393350.
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Hi Does SGLT2 antibody detect rabbit SGLT2?

Asked by: Azam
Thank you for your question. We have not tested this mouse monoclonal antibody, SGLT-2 (D-6): sc-393350, for reactivity in rabbit samples. The antibody is raised against amino acids 226-268 mapping within an internal region of SGLT-2 of human origin. Based on sequence analysis, the immunogen sequence does share 91% sequence identity with the published protein sequence for rabbit SGLT2 (see link below as a reference for that sequence). This is a very high degree of sequence identity, and there is a good chance that this antibody would detect rabbit SGLT-2. http://www.uniprot.org/uniprot/P26430
Answered by: Technical Support
Date published: 2017-10-04

I was wondering if this antibody reacts with mouse SGLT-2. They share 86% identity in the amino acid sequence used as a antigen. If not, could you recommend one for IHC/WB?

Asked by: Arrosung01
Thank you for your question. We have not yet tested this mouse monoclonal, SGLT-2 (D-6): sc-393350, for detection of SGLT-2 in mouse samples. Based on the sequence identity with the antigen, it is highly likely that this antibody will also detect the mouse protein. If you would be interested in testing a free 10 µg sample of SGLT-2 (D-6): sc-393350 for this application, please contact our Technical Service Department. Please call our Asian Support office toll-free at 800-820-8626, email asia@scbio.cn, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-25

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with sc-393350: SGLT-2 (D-6)?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-12

Does sc-393350 cross-react with SGLT-1?

Asked by: cjMara
sc-393350: SGLT-2 (D-6) is a mouse monoclonal antibody that is specific for SGLT-2. It is not expected to cross-react with SGLT-1.
Answered by: Technical Support 7
Date published: 2017-01-12
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