Anti-Sg II Antibody (SGII 9G3/3) is a mouse monoclonal IgG2b κ Sg II antibody, cited in 2 publications, provided at 200 µg/ml
raised against rat synthetic peptide
Anti-Sg II Antibody (SGII 9G3/3) is recommended for detection of secretogranin II of mouse and rat origin by WB, IP, IF and FCM
Anti-Sg II Antibody (SGII 9G3/3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Sg II (SGII 9G3/3): sc-53441.
m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for Sg II Antibody (SGII 9G3/3) for WB applications. These reagents are now offered in bundles with Sg II Antibody (SGII 9G3/3) (see ordering information below).
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Sg II Antibody (SGII 9G3/3) is a high quality monoclonal Sg II antibody (also designated Sg II antibody) suitable for the detection of the Sg II protein of mouse and rat origin. Sg II Antibody (SGII 9G3/3) is available as both the non-conjugated anti-Sg II antibody form, as well as multiple conjugated forms of anti-Sg II antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A); chromogranin B (Chr-B), also known as secretogranin I; chromogranin C (also known as secretogranin II or Sg II); and secretogranin III (Sg III or SCG3). High levels of Chr-A expression are characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Hi is this product replaceing SC-54327? Has been developed using full length peptide (33aa?)
Asked by: Dag Christiansen
Thank you for your question. Sg II Antibody (SGII 9G3/3): sc-53441 has been raised against amino acids 173-181 of rat origin (accession # P10362). For further information please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
How can Sg II (SGII 9G3/3): sc-53441 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?
Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53441 PE) or fluoroscein (sc-53441 FITC).
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