Anti-SerpinB8 Antibody (PI-8) recommended for detection of SerpinB8 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-SerpinB8 Antibody (PI-8): sc-101371

Anti-SerpinB8 Antibody (PI-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-SerpinB8 Antibody (PI-8) is a mouse monoclonal IgG1 κ SerpinB8 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against SerpinB8 of human origin
    • SerpinB8 Antibody (PI-8) is recommended for detection of SerpinB8 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SerpinB8 Antibody (PI-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SerpinB8 (PI-8): sc-101371.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for SerpinB8 Antibody (PI-8) for WB and IHC(P) applications. These reagents are now offered in bundles with SerpinB8 Antibody (PI-8) (see ordering information below).

Can SerpinB8 (PI-8): sc-101371 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, SerpinB8 (PI-8): sc-101371 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Westernblot Good quality Mab.Used it in WB,tried dilution 1:200 and 1:500, bot strong signal for human colon cell lines detection.
Date published: 2018-01-27
Rated 5 out of 5 by from Used for western blot analysis of SerpinB8 Used for western blot analysis of SerpinB8 expression in mucosa oropharynx, spleen, placenta, pituitary glad, epidermis, stomach, colon, lung, kidney, liver, heart, brain and pancreas human tissue lysates. Reproduced, with permission, from Strik MC, Bladergroen BA, Wouters D, Kisiel W, Hooijberg JH, Verlaan AR, Hordijk PL, Schneider P, Hack CE, Kummer JA: Distribution of the human intracellular serpin protease inhibitor 8 in human tissues. Journal of Histochemistry & Cytochemistry. 50(11):1443-54, 2002. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Works well for western blotting in human Works well for western blotting in human SerpinB8 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-06
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