Septin 5 Double Nickase Plasmid (h): sc-402514-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Septin 5 Double Nickase Plasmid (h) and Septin 5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEPT5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Septin 5 Antibody (SP18): sc-20040
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 5 Double Nickase Plasmid (h) | sc-402514-NIC | 20 µg | $410.00 |
Human SEPT5 encodes septin 5, a GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and contributes to membrane organization, vesicle trafficking, and spatial control of actin and microtubule dynamics. Septin 5 is enriched in neuronal and secretory contexts and has been linked to synaptic vesicle exocytosis and docking through interactions with SNARE-associated machinery. SEPT5-dependent septin scaffolds influence cell polarity, cytokinesis, and compartmentalization at the plasma membrane, processes that shape intracellular transport and signal propagation. Altered SEPT5 expression or locus disruption has been reported in neurodevelopmental and neuropsychiatric research contexts and in cancer-associated expression datasets, supporting its use as a mechanistic node in studies of cytoskeletal remodeling and secretion.
Septin 5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEPT5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEPT5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEPT5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEPT5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.