Septin 10 Antibody (C-3)recommended for detection of Septin 10 isoforms 1 and 2 of human origin by WB, IP, IF, IHC(P) and ELISA

Septin 10 Antibody (C-3): sc-365776

Septin 10 Antibody (C-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • Septin 10 Antibody (C-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-45 mapping at the N-terminus of Septin 10 of human origin
    • recommended for detection of Septin 10 isoforms 1 and 2 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can Septin 10 (C-3): sc-365776 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, Septin 10 (C-3): sc-365776 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Good Western Blot data of Septin 10 expression Good Western Blot data of Septin 10 expression in Caki-1 whole cell lysate. -SCBT QC
Date published: 2013-03-30
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