Anti-secretin receptor Antibody (E-9) recommended for detection of secretin precursor and active peptide of human origin by WB, IP, IF and ELISA

Anti-secretin receptor Antibody (E-9): sc-166112

Anti-secretin receptor Antibody (E-9) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-secretin receptor Antibody (E-9) is a mouse monoclonal IgG1 (kappa light chain) secretin receptor antibody provided at 200 µg/ml
    • raised against amino acids 33-121 mapping at the C-terminus of secretin of human origin
    • secretin receptor Antibody (E-9) is recommended for detection of secretin precursor and active peptide of human origin by WB, IP, IF and ELISA
    • Anti-secretin receptor Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of secretin receptor (E-9): sc-166112.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for secretin receptor Antibody (E-9) for WB applications. These reagents are now offered in bundles with secretin receptor Antibody (E-9) (see ordering information below).
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Is there any secretin antibody available specific for mouse/rat.If available can we inject this antibody by intra peritoneally/i.v./ in vivo in animal to check its binding to secretin receptor in vivo?

Asked by: vijay modak
Thank you for your question. At this time we have not validated any of our secretin receptor antibodies for reactivity with mouse or rat. We do not support any of our products for use in vivo or in live animal studies.
Answered by: Technical Service
Date published: 2017-04-24

For Western Blot, is it recommended to use denatured or non-denatured conditions with secretin receptor (E-9): sc-166112 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Doesn't recognize the secretin receptor This antibody does not recognize secretin receptor should be all ubiquitous in the gut villi epithelium but perhaps recognizes secretin.
Date published: 2021-04-09
Rated 4 out of 5 by from Good antibody Works as we expected. Tested by immunofluorescence.
Date published: 2018-12-18
Rated 4 out of 5 by from This antibody works In a western blot, the antibody worked well and thus we recommend it to others.
Date published: 2018-01-19
Rated 5 out of 5 by from Produced positive Western blot data of truncated Produced positive Western blot data of truncated human recombinant secretin fusion protein. -SCBT QC
Date published: 2014-05-08
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