Sec8 Antibody (B-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against amino acids 675-974 mapping at the C-terminus of Sec8 of human origin
Sec8 Antibody (B-11) is recommended for detection of Sec8 of mouse, rat and human origin by WB, IP, IF and ELISA
Sec8 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Sec8 (B-11): sc-514215.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Exocytosis is crucial in membrane trafficking and it mediates hormone and neurotransmitter secretion out of the cell, as well as the incorporation of membrane proteins and lipids to the plasma membrane. It is crucial for cell-cell communication, cell growth and cell polarity. The exocyst complex is a multi-protein complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84, and is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex inhibits tubulin polymerization in vitro, suggesting that the exocyst complex is important for modulating the microtubule dynamics that underlie exocytosis. Sec8, also known as EXOC4 (exocyst complex component 4), REC8 or SEC8L1, is one of eight protein subunits composing the mammalian exocyst complex. Human Sec8 maps to chromosome 7q33.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with Sec8 (B-11): sc-514215 monoclonal antibody for Western blot?
Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 4 out of
Good for IF and IHCThis Sec8 antibody shows strong specific signal in IF and IHC stainings in primary rat motor neuron.
Date published: 2017-11-06
Rated 5 out of
an excellent antibody for both WB and IFThis antibody recognizes a clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of
P Group from
Good for WesternsSuccessful detection of Sec8 in MDCK whole cell lysates - incubated @ 1:500 O/N at 5˚C.
Date published: 2017-07-19
Rated 5 out of
Satisfactory Western blot data of Sec8 expressionSatisfactory Western blot data of Sec8 expression in non-transfected 293T, human Sec8 transfected 293T HeLa and Jurkat whole cell lysates and HeLa nuclear extract . -SCBT QC
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