Sec31A Antibody (H-2)recommended for detection of Sec31A of human origin by WB, IP, IF and ELISA

Sec31A Antibody (H-2): sc-376587

Sec31A Antibody (H-2) is rated 4.9 out of 5 by 10.
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Datasheets

    • Sec31A Antibody (H-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 429-571 mapping within an internal region of Sec31A of human origin
    • Sec31A Antibody (H-2) is recommended for detection of Sec31A of human origin by WB, IP, IF and ELISA
    • Sec31A Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Sec31A (H-2): sc-376587.
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What is the recommend I.F dilution for this antibody?

Asked by: Richik
Thank you for your question. We recommend a starting dilution of 1:50 with this mouse monoclonal, Sec31A Antibody (H-2): sc-376587. The typical range of dilutions we recommend testing for optimization is 1:50-1:500.
Answered by: Technical Support
Date published: 2017-04-17

What is the recommended fixation method for immunofluorescence staining with Sec31A (H-2): sc-376587 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect antibody to COP-II vesicle marker (Sec31) I used this clone successfully for immunocytostaining, western blotting of human and mouse cell and tissues. For staining of cartilages this antibody totally co-localized with Sec23a which is also COP-II vesicle marker. So this antibody is very useful for multiple application.
Date published: 2019-08-28
Rated 5 out of 5 by from good for immunofluorescence It was used in immuofluorescence 1:100 on primary endothelial cells fixed by PFA
Date published: 2018-05-04
Rated 5 out of 5 by from sec31抗体很有效 做Western Blot很好,条带比较清晰,杂带很少,位是置也对,在130kd的位置。在INS1细胞和293T细胞中 做的
Date published: 2018-03-25
Rated 4 out of 5 by from Good Tested this antibody for IF at 1:50-75 dilution on PFA fixed HeLa cells. The results were fair enough.
Date published: 2017-05-05
Rated 5 out of 5 by from Specific COP-II vesicle staining with Sec23a It worked in both 4% PFA and cold MeOH-fixed cells and it perfectly colocalized with sec23a which is same COP-II component. It looked like dot like structures.
Date published: 2017-02-02
Rated 5 out of 5 by from Quality IF staining Bright and clear staining seen in IF with methanol fixed cells.
Date published: 2017-01-19
Rated 5 out of 5 by from IPs Recognizes Sec31 in HeLa and 293T cell lysate by WB. Bands are specific, as they go away with siRNA treatment. Antibody is also able to IP nicely.
Date published: 2016-12-06
Rated 5 out of 5 by from Used in WB Used in WB, correct MW band at 1:200 dilution.
Date published: 2015-06-08
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