RXR gamma Antibody (A-2)recommended for detection of RXRγ of human origin by WB, IP, IF and ELISA

RXRγ Antibody (A-2): sc-365252

RXRγ Antibody (A-2) is rated 4.8 out of 5 by 5.
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Datasheets

    • RXRγ Antibody (A-2) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-105 of RXRγ of human origin
    • RXR gamma Antibody (A-2) is recommended for detection of RXRγ of human origin by WB, IP, IF and ELISA
    • RXR gamma Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-365252 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RXRγ (A-2): sc-365252.
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Is this antibody predicted to work on mouse tissue?

Asked by: Liyam
Thank you for your question. Unfortunately this antibody has only been validated on human samples, and is not recommended or warrantied for use on mouse samples.
Answered by: Tech Service
Date published: 2019-01-16

What is the recommended fixation method for immunofluorescence staining with RXRγ (A-2): sc-365252 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great for WB It works great for WB mouse protein in tissue, 3% BSA in PBST, at 1:1000 dilution.
Date published: 2018-02-20
Rated 5 out of 5 by from great immunofluorescent staining it works great on the frozen section of retinal organoid.
Date published: 2017-08-25
Rated 4 out of 5 by from Works well with antigen retrieval Tried a sample of this monoclonal antibody and found it works well in our fixed frozen retinal sections.
Date published: 2017-07-26
Rated 5 out of 5 by from Exceptional immunofluorescence nuclear and Exceptional immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Satisfactory Western Blot data of RXR expression Satisfactory Western Blot data of RXR expression in non-transfected and human RXR transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-03-19
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