Anti-RXR alpha / beta / gamma Antibody (F-1) recommended for detection of RXRα, RXRβ and RXRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-RXRα/β/γ Antibody (F-1): sc-46659

Anti-RXRα/β/γ Antibody (F-1) is rated 4.5 out of 5 by 11.
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Datasheets

    • RXRα/β/γ Antibody (F-1) is a mouse monoclonal IgG1 κ, cited in 37 publications, provided at 200 µg/ml
    • raised against amino acids 198-462 of RXRα of human origin
    • Anti-RXR alpha / beta / gamma Antibody (F-1) is recommended for detection of RXRα, RXRβ and RXRγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-RXR alpha / beta / gamma Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-46659 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RXRα/β/γ (F-1): sc-46659.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RXRα/β/γ Antibody (F-1) for WB and IHC(P) applications. These reagents are now offered in bundles with RXRα/β/γ Antibody (F-1) (see ordering information below).
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do you have a citation with chip seq experiment with this ab please

Asked by: lina fadel
Thank you for your question. We are currently aware of 4 different product citations using this antibody for different ChIP applications. Please find these references on PubMed, or on our website using the following reference ID's, PMID's: 28534485, 20123735, 28534485 and 30601699.
Answered by: Technical Support
Date published: 2020-07-22

HI, I want to buy RXR antibody to do Chromatin immunoprecipitation (ChIP), I wonder whether this antibody (sc-46659) can recognize and bind to all three RXR isoforms (RXRα/β/γ), because I need ChIP for these three together. Thanks!

Asked by: Anonymous
Thank you for your question. Yes, this antibody is reactive with all three forms RXRα/β/γ.
Answered by: Tech Service
Date published: 2020-05-29

Can you please cite a publication in which this ab has been used susccessfully for ChIP?

Asked by: CharlesF
Thank you for your question. Yes, please refer to the following publication, PMID: 20123735, as a reference for using this antibody in ChIP.
Answered by: Technical Support
Date published: 2019-01-26

Can the X form of this antibody be used for applications other than ChIP or EMSA, such as Western Blot?

Asked by: AbPolly
Thank you for your question. Yes, the X form can be used in other applications including Western Blot. Be sure to adjust the dilution factor as the X form is more concentrated than the standard antibody.
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 4 out of 5 by from 探讨一下哦 用这个抗体做了人细胞的WB(1:200) ,出了三条带,大约40多KD ,50几KD,100左右还有一条,有人用的也是这样吗?交流一下
Date published: 2019-11-01
Rated 5 out of 5 by from very good antibody This antibody works very well in Wb. Also worked in IF in a PLA assay. Very satisfied.
Date published: 2019-01-02
Rated 5 out of 5 by from WB and IHC This antibody works i n WB and IHC of human tissue.
Date published: 2018-11-12
Rated 3 out of 5 by from RXR used for WB I applied for a sample two week ago.My sample is the mouse liver. Incubated the antibody for overnight at 4℃, 1:1000 dilution, and  m-IgGκ BP-HRP  incubated for 1 hour at RT , 1:1000 dilution.The results are shown in the figure.
Date published: 2017-09-16
Rated 5 out of 5 by from got target bands Incubated the antibody for 1hour at RT, 1:500 dilution, expose 30s is enought got the target bands at 52kda.
Date published: 2017-06-15
Rated 5 out of 5 by from worked well Specific signal in IF in human liver tissue, dilution: 1:50.
Date published: 2017-05-18
Rated 5 out of 5 by from work well in WB our lab bought RXR antibody in Santa , the quality is significant well known, I suggest!
Date published: 2017-03-08
Rated 5 out of 5 by from Great antibody Nice and specific WB and IHC (P) results with My Review for RXR alpha/beta/gamma Antibody (F-1)
Date published: 2017-01-12
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