Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RUNX3 Double Nickase Plasmid (m): sc-419487-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RUNX3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RUNX3 Double Nickase Plasmid (m) and RUNX3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Runx3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RUNX3 Antibody (A-3): sc-376591
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUNX3 Double Nickase Plasmid (m) | sc-419487-NIC | 20 µg | $410.00 | |||
RUNX3 Double Nickase Plasmid (m2) | sc-419487-NIC-2 | 20 µg | $410.00 |
Runx3 encodes the transcription factor RUNX3, a Runt domain DNA-binding protein that partners with CBFβ to regulate lineage commitment, differentiation, and tissue homeostasis. In mouse cells, RUNX3 integrates signals from TGF-β/SMAD and Wnt/β-catenin pathways to modulate transcriptional programs controlling cell-cycle progression, apoptosis, and immune cell development. Runx3 activity is particularly relevant to cytotoxic lymphocyte maturation and epithelial differentiation, where altered expression or function is associated with dysregulated proliferation and inflammatory phenotypes. These properties make Runx3 a useful node for dissecting transcriptional circuitry and signaling cross-talk in development and disease-relevant model systems.
RUNX3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Runx3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Runx3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Runx3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Runx3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.