Anti-RT1-Ac Antibody (OX27) recommended for detection of RT1-Ac of mouse and rat origin by IF and FCM

Anti-RT1-Ac Antibody (OX27): sc-53076

Anti-RT1-Ac Antibody (OX27) is rated 3.0 out of 5 by 1.
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Datasheets

    • Anti-RT1-Ac Antibody (OX27) is a mouse monoclonal IgG2a (kappa light chain) RT1-Ac antibody provided at 200 µg/ml
    • raised against PHA activated lymphocytes of rat origin
    • Anti-RT1-Ac Antibody (OX27) is recommended for detection of RT1-Ac of mouse and rat origin by IF and FCM
    • Anti-RT1-Ac Antibody (OX27) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RT1-Ac (OX27): sc-53076.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RT1-Ac Antibody (OX27) for WB and IHC(P) applications. These reagents are now offered in bundles with RT1-Ac Antibody (OX27) (see ordering information below).
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How can RT1-Ac (OX27): sc-53076 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53076 PE) or fluoroscein (sc-53076 FITC).
Answered by: Technical Support
Date published: 2017-03-01
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Rated 3 out of 5 by from Nice indirect FCM analysis of rat peripheral Nice indirect FCM analysis of rat peripheral blood leukocytes stained with HLA-DP . -SCBT QC
Date published: 2014-08-23
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