RSAD2 Antibody (G-8): sc-390342

RSAD2 Antibody (G-8) is a mouse monoclonal IgG3 kappa light chain antibody that detects RSAD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RSAD2 antibody (G-8) is available as the non-conjugated monoclonal isotype antibody. RSAD2, also known as Radical S-adenosyl methionine domain-containing protein 2, CIG5, vig1, viperin, or CIG33, is a 361 amino acid protein that plays a critical role in the antiviral defense mechanism of cells against various pathogens, including Hepatitis C, Cytomegalovirus, and HIV-1. RSAD2 is primarily localized to the cytosolic side of the endoplasmic reticulum, where RSAD2 is strategically positioned to respond to viral infections by relocating to the Golgi apparatus. This relocation is significant as RSAD2 disrupts lipid rafts at the plasma membrane, thereby preventing viral budding and supporting the interferon-induced antiviral state of the cell. RSAD2 has been shown to interact with and inactivate farnesyl diphosphate synthase (FDPS), an enzyme essential for cholesterol synthesis and the production of geranylated and farnesylated proteins, which are vital for various cellular functions. RSAD2 overexpression can lead to abnormal lipid accumulation, a condition associated with atherosclerosis, a chronic inflammatory disease characterized by the hardening of arteries.

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RSAD2 Antibody (G-8) References:

1. Viperin (cig5), an IFN-inducible antiviral protein directly induced by human cytomegalovirus.  |  Chin, KC. and Cresswell, P. 2001. Proc Natl Acad Sci U S A. 98: 15125-30. PMID: 11752458
2. The antiviral cytomegalovirus inducible gene 5/viperin is expressed in atherosclerosis and regulated by proinflammatory agents.  |  Olofsson, PS., et al. 2005. Arterioscler Thromb Vasc Biol. 25: e113-6. PMID: 15890971
3. Analysis of ISG expression in chronic hepatitis C identifies viperin as a potential antiviral effector.  |  Helbig, KJ., et al. 2005. Hepatology. 42: 702-10. PMID: 16108059
4. Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1.  |  Severa, M., et al. 2006. J Biol Chem. 281: 26188-95. PMID: 16849320
5. Astrocyte indoleamine 2,3-dioxygenase is induced by the TLR3 ligand poly(I:C): mechanism of induction and role in antiviral response.  |  Suh, HS., et al. 2007. J Virol. 81: 9838-50. PMID: 17626075
6. The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts.  |  Wang, X., et al. 2007. Cell Host Microbe. 2: 96-105. PMID: 18005724
7. Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus.  |  Jiang, D., et al. 2008. J Virol. 82: 1665-78. PMID: 18077728
8. A unifying view of the broad-spectrum antiviral activity of RSAD2 (viperin) based on its radical-SAM chemistry.  |  Honarmand Ebrahimi, K. 2018. Metallomics. 10: 539-552. PMID: 29568838
9. RSAD2 Is an Effective Target for High-Yield Vaccine Production in MDCK Cells.  |  Qiao, Z., et al. 2022. Viruses. 14: PMID: 36423196
10. RSAD2 is abundant in atherosclerotic plaques and promotes interferon-induced CXCR3-chemokines in human smooth muscle cells.  |  Hayderi, A., et al. 2024. Sci Rep. 14: 8196. PMID: 38589444
11. Crucial Roles of RSAD2/viperin in Immunomodulation, Mitochondrial Metabolism and Autoimmune Diseases.  |  Chen, S., et al. 2024. Inflammation.. PMID: 38909344
12. HIV-induced RSAD2/Viperin supports sustained infection of monocyte-derived macrophages.  |  Zankharia, U., et al. 2024. J Virol. 98: e0086324. PMID: 39258908

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RSAD2 Antibody (G-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RSAD2 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RSAD2 Antibody (G-8) Specifications

Alternate Product Name: RSAD2

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 126-138

Epitope Target: RSAD2

Epitope Species: human

Product Reactivity/Detection: RSAD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RSAD2 Antibody (G-8): P: Blocking Peptide