RPA194 Antibody (F-6)recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF and ELISA

RPA194 Antibody (F-6): sc-46699

RPA194 Antibody (F-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • RPA194 Antibody (F-6) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of RPA194 of human origin
    • recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with RPA194 (F-6): sc-46699 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from good antibody for IF specific nucleolar staining of PF-fixed U2OS cells (1:100). not tested in other applications yet
Date published: 2017-02-23
Rated 5 out of 5 by from Bright nucleolar localization of signal in Bright nucleolar localization of signal in IF with MeOH fixed HeLa cells -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from Strong expression seen in 293T and HeLa whole Strong expression seen in 293T and HeLa whole cell lysates in WB -SCBT QC
Date published: 2014-03-30
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