RPA194 Antibody (C-1)recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

RPA194 Antibody (C-1): sc-48385

RPA194 Antibody (C-1) is rated 4.5 out of 5 by 10.
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Datasheets

    • RPA194 Antibody (C-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of RPA194 of human origin
    • RPA194 Antibody (C-1) is recommended for detection of RPA194 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • RPA194 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RPA194 (C-1): sc-48385.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 4 out of 5 by from RPA194, sc-48385 Works for Western blotting for different cancer cell lines.
Date published: 2018-11-14
Rated 5 out of 5 by from an excellent antibody for IF This antibody gives a very good and clean signal for IF (1:200 dilution, formaldehyde-fixed HeLa).
Date published: 2017-07-09
Rated 2 out of 5 by from Used for immunostaining in mouse ES cells Looking for a nucleolar maker, I tried a sample of this antibody to co-localize within the nucleolar space. I did not observe the desired localization signal rather more dispersed staining signal.
Date published: 2017-06-05
Rated 5 out of 5 by from Nucleolar staining!!! Strong western blotting data and best ever nucleolar if staining; forget the polyclonal alternatives
Date published: 2016-12-31
Rated 5 out of 5 by from Works well in multiple applications Great RPA194 antibody for Western and cell/tissue staining, even with paraffin.
Date published: 2016-12-30
Rated 5 out of 5 by from Good antibody for WB and IP Good antibody for WB and IP- tested in mouse cell lines.
Date published: 2015-10-23
Rated 4 out of 5 by from Working well so far Working well so far.
Date published: 2015-10-19
Rated 5 out of 5 by from Strong nucleolar localization in methanol Strong nucleolar localization in methanol-fixed HeLa cells in IF. -SCBT QC
Date published: 2015-05-16
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