Anti-ROR gamma Antibody (D-4) recommended for detection of RORγ of human origin by WB, IP, IF and ELISA

Anti-RORγ Antibody (D-4): sc-365476

Anti-RORγ Antibody (D-4) is rated 4.0 out of 5 by 4.
  • y_2022, m_5, d_18, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_365476, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
loading
5
1
4
2
3
1
2
0
1
0

Datasheets

    • Anti-RORγ Antibody (D-4) is a mouse monoclonal IgG1 κ RORγ antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 131-320 mapping within an internal region of RORγ of human origin
    • ROR gamma Antibody (D-4) is recommended for detection of RORγ of human origin by WB, IP, IF and ELISA
    • Anti-ROR gamma Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-365476 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RORγ (D-4): sc-365476.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RORγ Antibody (D-4) for WB applications. These reagents are now offered in bundles with RORγ Antibody (D-4) (see ordering information below).
loading

What is the recommended starting dilution for Western blot for sc-365476: RORγ (D-4) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
  • y_2022, m_5, d_18, h_22CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_365476, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good antibody This antibody detected RORgamma with high sensitivity but showed some background proteins.
Date published: 2017-10-20
Rated 4 out of 5 by from great binds tissue: spleen and liver WB, 1:1000 diluted in 5%BSA(TBST), incubate 4℃ overnight, and IgG-HRP, RT, 1hour. liver tissue, two bind (55KD and 20KD)
Date published: 2017-09-17
Rated 3 out of 5 by from Good antibody The antibody works for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
Rated 5 out of 5 by from Satisfactory Western blot data of ROR expression Satisfactory Western blot data of ROR expression in non-transfected and human ROR transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-05-15
  • y_2022, m_5, d_18, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_en_US, sid_365476, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT