Rootletin Antibody (E-5)recommended for detection of Rootletin of human origin by WB, IP, IF and ELISA

Rootletin Antibody (E-5): sc-390720

Rootletin Antibody (E-5) is rated 5.0 out of 5 by 2.
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Datasheets

    • Rootletin Antibody (E-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1207-1290 mapping within an internal region of Rootletin of human origin
    • recommended for detection of Rootletin of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended starting dilution for Western blot for sc-390720: Rootletin (E-5) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Produced positive Western Blot data of Rootletin Produced positive Western Blot data of Rootletin expression in Y79 nuclear extract and Daoy whole cell lysate . -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Produced positive Western Blot data of Rootletin Produced positive Western Blot data of Rootletin expression in Y79 nuclear extract. -SCBT QC
Date published: 2013-01-04
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