Anti-Ron beta Antibody (E-3) recommended for detection of Ron β of human origin by WB, IP, IF and ELISA

Anti-Ron β Antibody (E-3): sc-74588

Anti-Ron β Antibody (E-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-Ron β Antibody (E-3) is a mouse monoclonal IgG1 κ Ron β antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 531-690 of the Ron precursor of human origin
    • Ron beta Antibody (E-3) is recommended for detection of Ron β of human origin by WB, IP, IF and ELISA
    • Anti-Ron beta Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ron β (E-3): sc-74588.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ron β Antibody (E-3) for WB applications. These reagents are now offered in bundles with Ron β Antibody (E-3) (see ordering information below).
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Is this antibody suit for mouse, are there any details that you could share to us

Asked by: Anonymous
Thank you for your question. Ron β Antibody (E-3): sc-74588 is recommended for detection of Ron β of human origin by WB, IP, IF and ELISA. We do not recommend this antibody for the detection of its epitope in murine samples. We do however offer another antibody specific against this target: Ron β Antibody (E-9): sc-374626 is recommended for detection of Ron β of mouse, rat and human origin by WB, IP, IF and ELISA. You can find the datasheet on this antibody's product page and our recommended protocols on the bottom of this page under 'Resources'. Do not hesitate to contact our technical service directly if you have any further questions.
Answered by: Technical Support Europe
Date published: 2019-04-01

Is it possible to obtain the antibody higher concentrated or without gelatine?

Asked by: Dr Gruselglatz
Thank you for your question. Yes, we can provide this antibody at a higher concentration without gelatin. Please contact our Special Orders Department to to request a quote for this antibody. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-02-23

How can Ron β (E-3): sc-74588 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Professor Griffin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-74588 HRP), phycoerythrin (sc-74588 PE), fluoroscein (sc-74588 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good Western Blot data of Ron b expression Good Western Blot data of Ron b expression in SW480 and T-47D whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Produced positive Western Blot data of Ron Produced positive Western Blot data of Ron b expression in HCT 116 whole cell lysate. -SCBT QC
Date published: 2013-11-02
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