Anti-Ron beta Antibody (A-8) recommended for detection of Ron β of human origin by WB, IP, IF and ELISA

Anti-Ron β Antibody (A-8): sc-74587

Anti-Ron β Antibody (A-8) is rated 5.0 out of 5 by 2.
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Datasheets

    • Ron β Antibody (A-8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 531-690 of the Ron precursor of human origin
    • recommended for detection of Ron β of human origin by WB, IP, IF and ELISA
    • See Ron β (E-3): sc-74588 for Ron β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ron β Antibody (A-8) for WB applications. These reagents are now offered in bundles with Ron β Antibody (A-8) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-74587: Ron β (A-8) monoclonal antibody?

Asked by: DefinitelyNotMatt
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Produced positive Western Blot data of Ron Produced positive Western Blot data of Ron b expression in SW480 and T-47D whole cell lysates. -SCBT QC
Date published: 2015-01-16
Rated 5 out of 5 by from Produced positive Western Blot data of Ron Produced positive Western Blot data of Ron b expression in HCT 116 whole cell lysate. -SCBT QC
Date published: 2013-11-04
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