Anti-Ron alpha Antibody (29) recommended for detection of Ron α of mouse, rat, human and canine origin by WB and IF

Anti-Ron α Antibody (29): sc-136060

Anti-Ron α Antibody (29) is rated 4.0 out of 5 by 2.
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Datasheets

    • Ron α Antibody (29) is a mouse monoclonal IgG2b provided at 50 µg/0.5 ml
    • raised against amino acids 40-224 of Ron α of human origin
    • recommended for detection of Ron α of mouse, rat, human and canine origin by WB and IF; not recommended for IP
    • See Ron α (C-5): sc-393523 for Ron α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ron α Antibody (29). This work is in progress.
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What is the recommended fixation method for immunofluorescence staining with Ron α (29): sc-136060 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane staining in HS 766T cells. -SCBT QC
Date published: 2015-03-05
Rated 4 out of 5 by from Strong band in WB in MDCK whole cell lysate Strong band in WB in MDCK whole cell lysate. -SCBT QC
Date published: 2015-01-21
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