Supplied as 5 vials of 250 μL.
RNA Sample Loading Buffer is formulated for use in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis. Formulated for use in Northern blots. This product is suitable for use in formaldehyde-agarose gel electrophoresis of RNA. It has been tested for RNase activity and none was detected. Free of Ethidium Bromide to optimize transfer efficiency.
Composition of RNA Sample Loading Buffer:
62.5% (v/v) deionized formamide
1.14 M formaldehyde
1.25X MOPS-EDTA-sodium acetate buffer
200 μg/ml bromophenol blue
200 μg/ml xylene cyanol FF
1. Rosen, K.M., et al. 1990. Biotechniques. 8: 398-403. PMID: 1692716
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