RNA Sample Loading Buffer, with EtBrA solution which promotes protein denaturation for use in SDS-PAGE, contains ethidium bromide

RNA Sample Loading Buffer, with EtBr

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Application: A solution which promotes protein denaturation for use in SDS-PAGE, contains ethidium bromide
* Refer to Certificate of Analysis for lot specific data (including water content).
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Each vial contains 250 μL.

RNA Sample Loading Buffer, with EtBr (ethidium bromide, 50µg/ml) is formulated for use in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required.
Note: This solution contains ethidium bromide which can interfere with transfer of RNA to nylon or nitrocellulose membranes.

Reagent Composition:
62.5 % deionized formamide
1.14 M formaldehyde
1.25X MOPS-EDTA-sodium acetate buffer
200 mg/ml bromophenol blue
200 mg/ml xylene cyanole FF
50 mg/ml ethidium bromide

Usage :
Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65°C for 10 minutes, and then chilled on ice immediately before loading on the gel.
Formulation :
Contains ethidium bromide (50 μg/ml)
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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