Rhodamine BA useful fluorochrome for histology, FRET and mitochondrial probe

Rhodamine B (CAS 81-88-9)

Rhodamine B | CAS 81-88-9 is rated 5.0 out of 5 by 1.
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Synonym: Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine
Application: A useful fluorochrome for histology, FRET and mitochondrial probe
CAS Number: 81-88-9
Purity: ≥95%
Molecular Weight: 479.01
Molecular Formula: C28H31N2O3•Cl
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Rhodamine B is a useful fluorochrome for histology due to its fluorescent properties in ultraviolet light. It ejects an electron onto TiO2, which is then scavenged by O2 forming the O2- radical anion. This anion ultimately becomes the OH radical. A pH-independent process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects. Rhodamine B has also been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration was precisely determined with the loss of fluorescence in the presence of MTT Formazan (sc-215399), a fluorescence quencher. In addition, Rhodamine B has been observed to significantly reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.


References

1. Kaji, T., et al. 1991. Toxicology. 68: 11-20. PMID: 1871776
2. Reungpatthanaphong, P., et al. 2003. J. Biochem. Biophys. Methods. 57: 1-16. PMID: 12834959
3. Di, W., et al. 2010. Nanotechnology. 21: 455703. PMID: 20947939

Usage :
Emission recorded at 545 nm
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (12 mg/ml), ethanol (70 mg/ml), acetone (slightly ), methyl cellosolve, and alcohol.
Storage :
Store at room temperature
Melting Point :
210-211° C (lit.)(dec.)
Density :
1.31 g/cm3
Fluoresence :
λex 554 nm, λem 627 nm in ethanol
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
BP3675000
PubChem CID :
6694
Merck Index :
14: 8183
MDL Number :
MFCD00011931
EC Number :
201-383-9
Beilstein Registry :
4119648
SMILES :
[Cl-].CCN(CC)C1=CC=C2C(OC3=C\\C(\\C=CC3=C2C2=C(C=CC=C2)C(O)=O)=[N+](\\CC)CC)=C1

Download SDS (MSDS)

Certificate of Analysis

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Rhodamine B  Product Citations

See how others have used Rhodamine B. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 28595966  Luzi, NM. et al. 2017. Anal. Biochem. 532: 45-52.

PMID: # 23024267  Ma, YY. et al. 2013. Carcinogenesis. 34: 68-78.

PMID: # 22232434  Ueno, H. et al. 2012. Invest. Ophthalmol. Vis. Sci. 53: 867-872.

PMID: # 27235784  Ugwu, MC. et al. J Pharmacol Toxicol Methods. 82: 9-19.

Citations 1 to 4 of 4 total

Por quanto tempo a Rhodamine B (CAS 81-88-9) emite fluorescência após aberto para uso? E qual a validade do produto?

Asked by: Anonymous
Thank you for your question. For accurate information regarding this biochemical product, please contact your local distributor for assistance.
Answered by: Tech Service
Date published: 2019-04-10
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Rated 5 out of 5 by from Rhodamine B is a commonly used fluorochrome Rhodamine B is a commonly used fluorochrome for histologic analysis that also serves as a FRET and mitochondrial probe and is cited in various publications. -SCBT Publication Review
Date published: 2015-02-25
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