Anti-Rev-erb alpha Antibody (E-12) recommended for detection of Rev-erbα of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Rev-erbα Antibody (E-12): sc-393215

Anti-Rev-erbα Antibody (E-12) is rated 4.5 out of 5 by 8.
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Datasheets

    • Anti-Rev-erbα Antibody (E-12) is a mouse monoclonal IgG1 κ Rev-erbα antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 21-69 mapping near the N-terminus of Rev-erbα of human origin
    • Anti-Rev-erb alpha Antibody (E-12) is recommended for detection of Rev-erbα of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Rev-erb alpha Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-393215 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rev-erbα (E-12): sc-393215.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rev-erbα Antibody (E-12) for WB applications. These reagents are now offered in bundles with Rev-erbα Antibody (E-12) (see ordering information below).
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I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-393215: Rev-erbα (E-12) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 3 out of 5 by from wb not good this antibody ! dilution used is1:500,except one nonspecial band at 30KD ,the band at 55KD not 80
Date published: 2017-11-15
Rated 5 out of 5 by from 效果很好! 条带很清晰,效果不错。但分子量略小于预测值,不知道是什么原因。另外,在25kd存在一条杂带,但不影响实验结果。
Date published: 2017-10-30
Rated 5 out of 5 by from Works well on mouse sample and placenta Works well on human placenta and mouse tissues.1/500 dilution was used for WB. All lane were very clear .
Date published: 2017-06-30
Rated 5 out of 5 by from Very Strong Signal in WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-03
Rated 3 out of 5 by from Specific signal in IHC application.....with Noise sc-393215 SAMPLE : Rev-erbα (E-12) Quite a bit of non-specific staining even at 1:500 dilution. It’s very difficult to find a rev-erb a antibody which works well in IHC.
Date published: 2016-12-30
Rated 5 out of 5 by from Got correct MW band in HEK293 cells Got correct MW band in HEK293 cells.
Date published: 2015-07-01
Rated 5 out of 5 by from Good Western blot data of Rev Good Western blot data of Rev-erb expression in non-transfected and mouse Rev-erb transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-10
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