Rent2 Antibody (G-10)recommended for detection of Rent2 of mouse, rat and human origin by WB, IP, IF and ELISA

Rent2 Antibody (G-10): sc-374230

Rent2 Antibody (G-10) is rated 4.5 out of 5 by 4.
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Datasheets

    • Rent2 Antibody (G-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 121-420 mapping within an internal region of Rent2 of human origin
    • Rent2 Antibody (G-10) is recommended for detection of Rent2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Rent2 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-374230 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rent2 (G-10): sc-374230.
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What is the recommended fixation method for immunofluorescence staining with Rent2 (G-10): sc-374230 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Detect it but with other background bands Used Rent2 at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. We were able to detect Rent2 but there were quite a few background bands as well.
Date published: 2017-09-29
Rated 5 out of 5 by from Great specificity and signal strength in Great specificity and signal strength in Y79 whole cell lysate via western blot. -SCBT QC
Date published: 2015-06-12
Rated 5 out of 5 by from Strong Strong, clean bands observed in western blot analysis of Rent2 expression in Ramos, Jurkat, Raji and HEK293 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-08
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