RECA-1 Antibody (RECA-1)recommended for detection of RECA-1 of mouse and rat origin by WB, IP and IF

RECA-1 Antibody (RECA-1): sc-52665

RECA-1 Antibody (RECA-1) is rated 4.0 out of 5 by 4.
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Datasheets

    • RECA-1 Antibody (RECA-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against peripheral and mesenteric lymph nodes of AO rat origin
    • recommended for detection of RECA-1 of mouse and rat origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RECA-1 (RECA-1): sc-52665.
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What is the recommended fixation method for immunofluorescence staining with RECA-1 (RECA-1): sc-52665 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-06-16
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Rated 4 out of 5 by from Buon prodotto Ho provato questo anticorpo in immunofluorescenza, su fettine di midollo spinale di topo criostatate. Diluizione 1:100, funziona bene, il segnale è pulito e specifico. Prossimamente lo proverò anche in WB
Date published: 2019-09-18
Rated 4 out of 5 by from IHC using frozen section of rat tissue We performed immunohistochemistry using the antibody and the frozen section of rat tissue fixed by 4% PFA. As a results, we can get specific positive signals by the antibody. Very good.
Date published: 2018-01-19
Rated 4 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for IF on rat brain - SCBT Publication Review
Date published: 2015-01-18
Rated 4 out of 5 by from IF publishable data with rat hepatic stellate IF publishable data with rat hepatic stellate cells - SCBT Publication Review
Date published: 2013-11-01
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