Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12) recommended for detection of RARβ2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12): sc-514585

Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12) is rated 4.5 out of 5 by 2.
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Datasheets

    • Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12) is a mouse monoclonal IgG1 κ RARβ/Retinoic Acid Receptor β antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-55 mapping at the N-terminus of RARβ2 of human origin
    • Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12) is recommended for detection of RARβ2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-RARβ/Retinoic Acid Receptor β Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-514585 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RARβ/Retinoic Acid Receptor β (B-12): sc-514585.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RARβ/Retinoic Acid Receptor β Antibody (B-12) for WB applications. These reagents are now offered in bundles with RARβ/Retinoic Acid Receptor β Antibody (B-12) (see ordering information below).
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How can RARβ2 (B-12): sc-514585 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-514585 HRP), phycoerythrin (sc-514585 PE), fluoroscein (sc-514585 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Great! I received this as a free sample. I used it at a 1:1000 dilution, and the secondary GAM at 1:1000 dilution on mouse and rat liver homogenates that were run on a 8% gel transferred to nitrocellulose membrane. The antibody was clean.
Date published: 2017-09-26
Rated 5 out of 5 by from this antibody works good for WB this antibody works good to detect DC samples at 1:400 dilution
Date published: 2017-09-22
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