Anti-RARα/Retinoic Acid Receptor α Antibody (1C10) recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-RARα/Retinoic Acid Receptor α Antibody (1C10): sc-293417

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Datasheets

    • RARα/Retinoic Acid Receptor α Antibody (1C10) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 100 µg/ml
    • raised against amino acids 315-424 representing partial length RARα of human origin
    • recommended for detection of RARα of mouse, rat and human origin by WB, IP, IF and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RARα/Retinoic Acid Receptor α Antibody (1C10). This work is in progress.
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Hi, Can I use this antibody to detect native RARa, eg. for antibody supershift gel shift assays?

Asked by: Mabelh
This antibody has not yet been tested for gel supershift (EMSA) and so this is not a recommended application at this time. The antibody is recommended for detection of native RAR alpha by IP and IF.
Answered by: Technical Support
Date published: 2018-12-21

I am using RARα (1C10): sc-293417 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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