Anti-RANKL Antibody (G-1) recommended for detection of RANKL of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-RANKL Antibody (G-1): sc-377079

Anti-RANKL Antibody (G-1) is rated 4.3 out of 5 by 14.
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Datasheets

    • Anti-RANKL Antibody (G-1) is a mouse monoclonal IgG1 κ RANKL antibody, cited in 33 publications, provided at 200 µg/ml
    • raised against amino acids 46-317 representing full length RANKL of human origin
    • Anti-RANKL Antibody (G-1) is recommended for detection of RANKL of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-RANKL Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RANKL (G-1): sc-377079.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RANKL Antibody (G-1) for WB applications. These reagents are now offered in bundles with RANKL Antibody (G-1) (see ordering information below).
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Hello! I purshed conjugate antibody aganist RANKL 377079HRP in order to detect RANKL in a bone sample by IHC(P) and I followed the immunoperoxidase staining protocol that you provide on-line. However, the processing is not working. Can you help me?

Asked by: Valesca
Thank you for your question. Please reach out to your local distributor directly for assistance troubleshooting your IHC results. They will be happy to help troubleshoot and discuss your results with you, and will work with Santa Cruz as needed.
Answered by: Tech Service
Date published: 2019-08-06

Does it work well on primary cell lysates?

Asked by: flowersf
Thank you for your question. RANKL Antibody (G-1): sc-377079 is recommended for detection of RANKL of mouse, rat and human origin by WB, IP, IF and ELISA. This recommendation is valid for lysates generated from both cell cultures and tissue material.
Answered by: Tech Support Europe
Date published: 2019-07-09

Boa Tarde! Nos comentários vocês não indicam IHQ para bloco parafinado, porém nas especificações conta isso "disponível conjugado a agarose para IP; HRP para WB, IHC (P) e ELISA; e para ou ficoeritrina ou FITC para IF, IHC (P) e FCM" Estou com duvidas!

Asked by: Janaína
Thank you for your question. The conjugated form of this antibody is recommended for IHC on paraffin sections, however, the non-conjugated form has been warrantied for IF on fresh frozen tissue sections.
Answered by: Tech Service
Date published: 2019-06-20

Which is the actual molecular weight of RANKL? the data sheet says 40-35 Kd, but I have got bands around 50Kd when using primary clavaria cells from mice...

Asked by: MP03
Thank you for your question. The expected molecular weight of RANKL, as detected by RANKL Antibody (G-1): sc-377079, depends on the form present in your sample: RANKL full-length (35-40 kDa), membrane-bound RANKL (34-40 kDa), or soluble RANKL (20-30 kDa) . If you are detecting a signal at a different height, please contact our technical service for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

What's the difference between the sc-52950 and the sc-377079 HRP in IHC with mouse tissue? And what about the result?Will they be same? 

Asked by: sumer
Thank you for your inquiry. Both sc-52950 and sc-377079 are mouse monoclonal antibodies recommended for detection of RANKL of mouse, rat and human origin. sc-52950 can be used on paraffin-embedded tissues, while sc-377079 is not recommended for such application. sc-377079 HRP is sc-377079 directly conjugated to HRP. When using sc-377079 HRP in WB or IHC, the secondary reagent can be avoided. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website. Best of luck with your research!
Answered by: Technical Support 14
Date published: 2017-07-10

How much protein do you recommend loading on the gel for Western blot detection using RANKL (G-1): sc-377079 monoclonal antibody?

Asked by: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-02-02
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Rated 3 out of 5 by from RANKL - Dot Blot RANKL Antibody works also for Dot Blot. The Antibody have been diluted 1:1000 in TBS-T buffer.
Date published: 2019-11-04
Rated 3 out of 5 by from RAW 264.7 Hard to work in the WB of osteoclast induced from RAW 264.7,with 1:500.
Date published: 2017-10-23
Rated 4 out of 5 by from Good bands This antibody works fine on western blot in 1:500. The bands are clear.
Date published: 2017-08-25
Rated 5 out of 5 by from good performance I used it for IF on HeLa cells (1:300 dilution). It works both on PFA and methanol-fixed cells.
Date published: 2017-05-28
Rated 5 out of 5 by from specific band Used this antibody to make WB in 1:600 dilution, we got obtained specific band.
Date published: 2017-05-27
Rated 5 out of 5 by from strong bands. Strong bands detected in WB for human lung tissue, use dilution 1:400.Recommend use it.
Date published: 2017-02-09
Rated 5 out of 5 by from recombinant RANKL It works great in WB, favorable band achieved in mouse recombinant RANKL.
Date published: 2017-02-07
Rated 1 out of 5 by from Cannot be used for paraffin immunohistology This antibody ist not applicable for immunohistology in formol-fixed paraffin sections using proteolytic, high temperature or no antigen retrieval.
Date published: 2017-01-18
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